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PartOligo 1Oligo 2TemplateSizeRunTreatmentVectorEnzymesSizesProduct

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Aca1067F ca1067RpSB1AK3-b0015 10552K55EcoRI/SpeI pSB1A2-I13521 EcoRI/SpeI 2062+946 LpSB1A2Bca9128[KanR]

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GFPBca1123Fca1123RpSB1A2-I1352274855BglII/XhoIpBca1102BglII/XhoI2159+697LpBca1102Bca1123{GFPmut3b}

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TrrnBCca1124Fca1124RpBAC98718955BglII/XhoIpBca9145-Bca1089BglII/XhoI2063+696LpBca9145Bca1124{TrrnB}

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cmRDca1121Fca1121RpSB1AC391155EcoRI/BglIIpBca1100-Bca1091EcoRI/BglII2987+9LpBca1121E-CmR-Bg-{Ptet}-Ba-rbsRFP

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kanREca1122Fca1122RpSB1AK3104355EcoRI/BglIIpBca1100-Bca1091EcoRI/BglII2987+9LpBca1122E-KnR-Bg-{Ptet}-Ba-rbsRFP

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periplasm-*Fca1125Fca1125RMG1655 gen.9855BglII/XhoIpBca1102BglII/XhoI2159+697LpBca1102Bca1125{fimH-prepro1}

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phoAGca1126Fca1126RpSB1A2-Bca103213852K55BglII/XhoIpBca9145-Bca1089BglII/XhoI2063+696LpBca9145Bca1126{phoA active peptide}