.

BRB80 (80 mM PIPES pH 6.9; 1 mM EGTA; 1 mM MgCl2)

.

.

Desired Volume of bufferL0.5

.

Concentration to store (e.g. "10x")1

.

.

Acid PIPES solid (FW 302.37)Desired Conc. Of bufferM0.08

.

(FLUKA 60369)FWg / mol302.37

.

grams solid to addg12.095

.

.

Acid EGTA solid (FW 380.35)Desired Conc. Of bufferM0.001

.

FWg / mol380.35

.

grams solid to addg0.190

.

.

MgCl2 solid (FW 203.30)Desired Conc. Of bufferM0.001

.

FWg / mol203.3

.

grams solid to addg0.102

.

.

H2OH2O to add80% first

.

.

KOH pelletsrough Conc. Needed for pH 6.8M0.08

.

(Fisher P250-500)FWg / mol56.11

.

grams solid to addg2.244