.

Total reaction mixdesired conc. (per 100 microliter)100.00100.00100.00100.00100.001001.05

.

Tube label123456MASTER

.

DI water (Diego)69.0069.0069.0069.0069.0069.00434.70

.

10X PCR Buffer, No Mg10.0010.0010.0010.0010.0010.0010.0063.00

.

July 2004 Miniprep #1, 1:100 (1.5 ng / ul)1.001.001.001.001.001.001.006.30

.

10 mM complete dNTP mix (From Kelly)2.502.502.502.502.502.502.5015.75

.

pRL574-F853-dig 10 µM 5.005.005.005.00

.

pRL574-F834-dig 10 uM5.005.005.005.00

.

pRL574-R5263-dig 10 uM5.005.005.00

.

pRL574-R2044-bio 10 uM5.005.005.00

.

pRL574-R2008-bio 10 uM5.005.005.00

.

50 mM MgCl26.506.506.506.506.506.506.5040.95

.

Taq (?U/µl) from Kelly1.001.001.001.001.001.001.006.30

.

MASTER90.0090.0090.0090.0090.0090.00567.00

.

.

.

.

Expected fragment sizes

.

2044.002008.005263.00

.

8531192.001156.004411.00

.

8341211.001175.004430.00

.