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Instrument:S/N 4325582

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Application:Fixed Wavelength Mode (Data)

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Created on:29 Aug 2003 11:42

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Base Directory:c:\data\spectrophotometer dataMolecular Weight90 kd

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App Directory:FIX_DATA

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Filename:082903SK.DUFEnzyme TypeConcentrationMolarity

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Elution #33.040 MG / Ml33.78 uM7/29/03 Bradford

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Mode:Abs

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Factor:No

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Number of wavelengths:2

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Wavelength

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650.0

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660.0

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This File:030829_Malachite Green Assay_Long-term trials.xls

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Sample IDAbs Abs

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650 nm 660 nm Quad. Fit [1]Pi CalibrationPi - MTDilution fact.molarityspecific activity

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8.29E-07(Note: different)

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zero0.00460.00377.62E-04

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zero0.00670.00586.20E-02

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00.09850.06767.4

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250.11350.080223.4

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500.13620.099346.6

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1000.18280.139892.7

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2000.31000.2535205.4

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4000.57020.4993(Linear fit)399.9

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5000.72530.64971.03E-03500.0 [2]

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10001.25461.16611.33E-011000.0

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T1 Full 1:4, 8/27/030.97000.8877730.5 [2]-34.9

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T1 Full 1:4, 8/29/030.40870.3435282.6326.7 [3]42230.007998526587208

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T1 C1 1:3, 8/27/031.23351.1512985.6 [2]42230.007998526587208#NAME?

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T1 C1 1:3, 8/29/030.39000.3256267.9

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T1 C2 1:4, 8/27/033.11233.05802831.8 [2]4220.421940928270.00800341251433#NAME?

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T1 C2 1:4, 8/29/030.71890.6445496.4158.8 [4]4220.421940928270.00800341251433#NAME?

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T2 Full 1:2, 8/27/031.50921.40961235.8 [2]

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T2 Full 1:4, 8/29/030.31390.2556207.3111.8 [3]42230.007998526587208

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T2 C 1:2, 8/27/031.67361.56171383.0 [2]

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T2 C 1:3, 8/29/030.35330.2916239.1

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Dial2 Full, 8/27/032.06201.94891757.9 [2]

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Dial2 Full, 8/29/030.27710.2238177.958.3 [3]4220.421940928270.00800341251433

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Dial2 C, 8/27/031.98491.87471686.1 [2]

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Dial2 C, 8/29/030.20980.1650119.7

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NOTE: All 8/27/03 Samples got screwed up mysteriously!!!!
[1] sjkoch: y = 8.2858E-07x2 + 7.6226E-04x + 6.1961E-02
[2] sjkoch: Linear fit 500,1000 because of dye precipitation above 500
[3] sjkoch: Adjusted for 1:3 & 1:4 dilutions
[4] sjkoch: This is Pi generation in the absence of MTs, adjusted from 25 mM ATP to 2 mM ATP